Evaluation of the efficiency of Trichoderma harzianum and Azotobacter chroococcum in Biodegradation of Glyphosate and measurement of its residual in soil by using HPLC

Abstract

This study was carried out in the laboratories and the agricultural station of the College of Agriculture at the University of Karbala for the purpose of evaluating the efficiency of Trichoderma harzianum and Azotobacter chroococcum in the biodegradation of glyphosate and measuring its residual in the soil by using the HPLC. Three herbicide concentrations at the dosage rate recommended were used, and the soil samples were taken immediately after the treatment and after 3, 7, 10, and 15 days of the treatment. The results showed that the three concentrations used 10, 15 and 20 ml / liter of water achieved  1552, 2235, 3096 and 1132, 1955, 2626 mg L-¹ for each fungus and bacteria, respectively, after 3 days of treatment, compared to the comparison treatment, which recorded 3104 and 4840 , 5582 mg L-1, and the control treatment, which recorded 3104, 4840, 5582 mg L-¹. On the 7th day, the concentrations decreased, recording 975, 1227, 1970 and 541, 942, 1242 mg L-¹ respectively, compared to the control treatment, which recorded 1493, 3675 and 4536 mg L-¹.The results of the study showed the ability of the fungus T. harzianum and the bacteria A. chroococcum In the biodegradation of glyphosate, there were no residual herbicides at the concentrations used in the study after 10 days of treatment.