Study of virulence factors and inhibitory effect of salvia on identified gram positive and gram negative isolated from soil

Abstract

Soil is considered one of the most suitable environmental for microbial growth . In this study and 130 bacterial isolates were from soil in musian city the first most common ratio was Escherichia .coli 30 (23.07) % and less of occurrence ratio was Citrobacter freundii 1(0.76) % also study some virulence factors like heamolysin the high ratio was Enterobacter spp 10(4)% and low ratio in Citrobacter freundii 1(1)% , protease was 9(27)% and Actinomycetes spp and Erwinia spp 0% finally in bacitracin was 8(32)% E.coli . Aqueous suspensions of the dried Allium sativum (Liliaceae) bulbs extract was screened for its antimicrobial activity using the agar-well diffusion method. The highest zone of inhibition was estimated with the highest concentration of aqueous suspension in Bacillus cereus was (17 mm) followed by lower inhibition zone in Enteroabacter cloacae was (5mm). It is tested against Gram-positive bacteria and gram-negative bacteria .The suspensions were tested at concentrations of 1, 10, 100 and 1000 μg/ml when using garlic extract as antimicrobial substances, salvia as inhibition factor, and inhabited zone of saliva in Micrococcus spp was 10 mm. Also inhibited adhesion bacterial on epithelial cell by saliva was E.coli group B from 68.5±30.3 to 40.2±18.1 and in group B Staphylococcus aureus inhibited bacteria on epithelial cell from 50.4±1.4 to 30.3±9.3 , conclusion soil contain many bacteria have some virulence factor and use saliva as inhibited adhesion factors